Reproducible RNA-seq analysis using recount2

Play Video

$2,000

Prizes

4,866

Views

1. Background

RNA sequencing (RNA-seq) is used to measure gene expression levels across the transcriptome for a huge variety of samples. For example, RNA-seq has been applied to

study gene expression in individuals with rare diseases, in hard-to-obtain tissues or for rare

forms of cancer. Many analyses require quantifying expressed features, which is a computationally intensive processing step. Deposited data are provided as raw sequencing reads, which are costly for standard academic labs researchers to analyze. We developed the recount2 project as our effort to standardize and share ready-to-analyze summarizations of RNA-seq data.


2. Method

Using the splice-aware Rail-RNA aligner, we processed over 70,000 human RNA-seq samples publicly available from the Sequence Read Archive, the Genotype-Tissue Expression (GTEx v6) and The Cancer Genome Atlas (TCGA) studies. Using the coverage bigWig files that Rail-RNA produces, we quantified gene and exons from the Gencode v25 database with the bwtool software. Additionally, we processed the exon-exon junction count data from Rail-RNA to provide ready-to-analyze count tables for these three expression features in the form of RangedSummarizedExperiment objects and text tables. Coverage bigWigs can also be used to determine expressed regions using the derfinder method. The expressed regions and the exon-exon junction count data are two annotation-agnostic methods that exemplify how the data in recount2 can be used for new analytical methods. The data are available via https://jhubiostatistics.shinyapps.io/recount/ and the recount Bioconductor package http://bioconductor.org/packages/recount.

3. Results

We compared the re-processed data in recount2 with the publicly available data from the GTEx project to demonstrate that our processing pipeline produced gene counts similar to other published methods. For protein-coding genes, the gene expression levels that we estimated using the recount2 pipeline had a median correlation of 0.987 with the GTEx v6 data. A differential expression analysis comparing colon and whole blood samples using the gene expression measurements from recount2 matched the results obtained using the v6 release from the GTEx portal (0.92 r2 for protein coding genes fold changes). We illustrated how recount2 can be used to investigate or validate cross-tissue differences using publicly available data by comparing samples from colon and whole blood from healthy individuals using a total of 5 studies. We compared the gene-level differential expression analysis results against results from applying the same models using data from GTEx and found that approximately 20% of the top 100 genes from the two analyses were concordant, which is higher than what is observed comparing lung and colon GTEx data (5% concordance). We provided an example on how data in recount2 can be used to evaluate expression differences across different feature-levels: genes, exons, junctions and expressed regions.

4. Conclusions

The recount2 project can be used for querying, downloading and analyzing large-scale human RNA-seq datasets across more than 70,000 samples, including all of GTEx, TCGA and the SRA. By removing a large number of data processing and quantification choices potentially made by researchers, recount2 reduces the number of “researcher degrees of freedom,” which can improve replication and reduce the potential for false positives created by processing

pipeline differences. By providing an updateable resource of uniformly processed RNA-seq samples, together with R-based software for analysis, recount2 will enable studies that individual laboratories would otherwise not have the resources to undertake.



5. Future ideas/collaborators needed to advance research?

We are always looking for new collaborators. You can find recent related projects to recount2 at https://jhubiostatistics.shinyapps.io/recount/ and if you are interested in doing a postdoc please get in touch with any of the principal investigators of the project. There is more information at the end of the video available at https://lcolladotor.github.io/biopeerprize2018/.

6. A link to your paper

https://f1000research.com/articles/6-1558/v1 (paper for competition)
https://doi.org/10.1038/nbt.3838 (related paper)

Comments

6
Javier De Las Rivas
8 months ago

An excellent piece of work.
Javier

Emiliano Barreto-Hernández
8 months ago

An excellent tool

Roderic Guigo
8 months ago

Great work. and of great utility. The need for uniformly processed RNASeq datasets is essential for large scale transcriptome comparisons

xiaojun xiaojun
4 months ago

20180622xiaoke

nhl jerseys wholesale


ugg outlet


kate spade handbags


coach outlet store online


ray ban sunglasses outlet


ralph lauren polo shirts


furla handbags


cheap oakley sunglasses


canada goose


coach factory outlet


kate spade purses


polo ralph lauren


pandora jewelry


hermes online


guess factory


under armour outlet


polo ralph lauren outlet


ugg boots clearance


off white outlet


10 deep clothing


jordan shoes


coach outlet online


jordan shoes


coach factory outlet


coach factory outlet


michael kors outlet online


polo ralph lauren outlet


christian louboutin sale


broncos jerseys


michael kors outlet clearance


coach outlet


nfl jerseys


pandora outlet


baltimore ravens jersey


polo ralph lauren outlet online


champion clothing


coach outlet store online


canada goose outlet


christian louboutin


michael kors outlet clearance


nfl jerseys wholesale


balenciaga sandals


harry winston jewelry


cheap ray ban sunglasses


canada goose outlet online


coach handbags online outlet


pandora jewelry


ralph lauren outlet online


barbour jackets outlet


cheap jordans


pandora charms sale clearance


ugg outlet store


canada goose outlet online


cheap jordan shoes


coach outlet online


christian louboutin


coach outlet store


coach factory outlet


swarovski crystal


cheap uggs


michael kors outlet clearance


michael kors outlet online


adidas crazy


pandora jewelry


michael kors outlet clearance


coach outlet store online


coach factory outlet


oakley sunglasses wholesale


ugg boots for women


ugg outlet


kd 9


michael kors outlet online


air max 90


christian louboutin


michael kors handbags


coach outlet store online


longchamp solde


ugg boots


polo outlet


malone souliers


coach outlet store online


christian louboutin sale


canada goose outlet store


lacoste pas cher


christian louboutin sale


tory burch outlet store


fendi handbags


coach outlet store


nfl jersey wholesale


canada goose uk


coach factory outlet


chloe sunglassess


cheap oakley sunglasses


longchamp pliage


michael kors outlet store


true religion jeans for men


ugg boots


coach factory outlet online


ugg boots on sale


kate spade outlet online


canada goose uk


ugg boots on sale


cheap jordan shoes


ray ban sunglasses outlet


michael kors outlet online


christian louboutin


tory burch handbags


michael kors outlet clearance


coach outlet


fossil watches


cheap air jordans


michael kors outlet clearance


bape clothing


ugg boots


oakley sunglasses wholesale


polo ralph lauren


nike pegasus


adidas shoes


ugg boots


michael kors outlet clearance


ralph lauren polo


cheap jordan shoes


coach factory outlet


freshjive clothing


kate spade handbags


g-star jeans


moncler outlet


bulgari jewelry


cheap jordan shoes


polo ralph lauren outlet


san antonio spurs jersey


mbt shoes outlet


kd 10 elite


ugg boots clearance


michael kors factory outlet


pandora outlet


cheap oakley sunglasses


pandora jewelry


polo ralph lauren shirts


polo pas cher


swarovski jewellery


colts jersey


cheap jordan shoes


kate spade outlet online


bcbg dresses


cheap ray ban sunglasses


brequet wathes


burberry outlet stores


uggs outlet online


coach outlet online


true religion jeans sale


adidas outlet store


carrera sunglasses


uggs outlet


broncos jersey


pandora outlet


ugg outlet store


cheap ray bans


canada goose uk


canada goose jackets sale


karen millen dresses


coach outlet store online


michael kors uk


coach factory outlet


coach outlet store online


air jordan retro


tory burch sandals


ralph lauren


canada goose outlet


canada goose coats


canada goose outlet store


coach factory outlet


christian louboutin


coach outlet online


cheap ray ban sunglasses


coach outlet store online


oakley sunglasses wholesale


kate spade new york


oakley sunglasses wholesale


canada goose outlet


ugg boots clearance


kate spade


ugg outlet online


michael kors outlet clearance


coach factory outlet


uggs outlet online


canada goose outlet online


pandora outlet


kate spade


polo outlet online store


pandora outlet


canada goose outlet sale


michael kors outlet clearance


ray-ban sunglasses


canada goose outlet online


longchamp outlet online


isabel marant outlet


michael kors online


miu miu outlet


kate spade purses


coach factory outlet


golden state warriors jersey


moncler


nhl jerseys for sale


coach factory outlet


barbour mens jackets


canada goose jackets


kate spade new york


uggs outlet online


kate spade outlet store


ralph lauren factory store


cheap air jordans


oakley sunglasses wholesale


coach outlet store online


ugg boots outlet


cheap jordan shoes


ugg outlet


basketball jerseys


air max 90


cheap oakley sunglasses


cheap jordans


cheap oakley sunglasses


polo ralph lauren outlet online


pandora jewelry


michael kors uk


chrome hearts sunglasses


michael kors outlet clearance


jordan shoes


a bathing ape


canada goose jackets clearance


jerseys from china


christian louboutin sale


balmain jeans


michael kors outlet


ray ban sunglasses sale


canada goose jackets


cheap jordans


dsquared2 jeans


ugg boots


cheap ugg boots


polo ralph lauren factory store


kate spade handbags


louboutin shoes


ray ban sunglasses outlet


manolo blahnik


ralph lauren outlet


coach outlet store online


nba jerseys wholesale


nike outlet


coach factory outlet


supreme clothing uk


kate spade


true religion outlet store


fitflops shoes


polo ralph lauren outlet online


cheap jordan shoes


michael kors outlet clearance


prada shoes on sale


uggs outlet


kevin durant jerseys


kate spade outlet store


belstaff jackets


pandora jewelry


michael kors uk


cheap ray ban sunglasses


ray ban sunglasses outlet


ralph lauren polo


coach factory outlet


michael kors outlet


ralph lauren


coach factory outlet


longchamp pas cher


michael kors outlet store


james harden jerseys


coach outlet canada


cheap air jordans


cheap jordans


ugg boots


canada goose


michael kors outlet clearance


pandora charms outlet


denver broncos jersey


light up shoes


canada goose


cheap air jordans


michael kors outlet


jordan shoes


christian louboutin


michael kors outlet clearance


adidas shoes


cheap ray ban sunglasses


nike outlet online


ralph lauren polo outlet


alexander mcqueen shoes


pandora charms


uggs outlet online


air max 97


tag heuer watches


ray ban sunglasses on sale


ugg outlet online


oakley sunglasses wholesale


michael kors outlet clearance


michael kors handbags


jordan shoes


canada goose outlet store


cheap air jordans


michael kors outlet clearance


michael kors bags


jerseys wholesale


uggs on sale


canada goose jackets


true religion jeans sale


brequet wathes


ugg boots clearance


pandora outlet


coach factory outlet


canada goose jackets


michael kors outlet clearance


air more uptempo


kate spade new york


michael kors outlet online


polo ralph lauren outlet online


diesel jeans


arcteryx jacket


ralph lauren shirts


cheap jordan shoes


cheap oakley sunglasses


ugg outlet online


cheap jordan shoes


coach outlet


mulberry bags


nike trainers


canada goose outlet store


air jordan shoes


cheap jordan shoes


chrome hearts clothing


canada goose outlet store


nike factory shoes


canada goose jackets


calvin klein jeans


air max 1


air jordan shoes


canada goose outlet store


canada goose


kyrie 4


coach factory outlet


isabel marant shoes


oakley sunglasses wholesale


michael kors outlet clearance


ralph lauren outlet


cheap jordans


suicoke sandals


ralph lauren outlet


harden vol 2


cheap jordans


coach outlet store online


coach factory outlet online


chopard jewelry


air max 2018


adidas soccer shoes


coach outlet online


polo ralph lauren outlet online


moncler coats


polo ralph lauren shirts


supreme clothing


ralph lauren outlet


jordan shoes


nike outlet store


chloe outlet


michael kors outlet clearance


kate spade new york


uggs outlet online


polo ralph lauren outlet


polo ralph lauren shirts


cheap ugg boots


polo outlet


kate spade


polo ralph lauren shirts


canada goose outlet store


air jordan shoes


stuart weitzman shoes


coach factory outlet


ralph lauren


alife clothing


coach outlet store online


nike shoes on sale


polo ralph lauren outlet


air jordan shoes


burberry outlet sale online


cheap jordan shoes


ray ban outlet


canada goose jackets for women


jordan 32


kevin durant shoes


canada goose jackets outlet


adidas outlet store


michael kors outlet clearance


mlb jerseys wholesale


cheap jordan shoes


pandora jewelry


pandora jewelry outlet


jimmy choo shoes


ugg outlet online


coach outlet store


ray ban sunglasses outlet


canada goose


coach outlet store


kate spade purses


pandora charms


cheap oakley sunglasses


true religion outlet store


houston texans jersey


michael kors outlet clearance


pandora jewelry


coach factory outlet


ugg outlet,ugg outlet stores


canada goose outlet online


oakley sunglasses wholesale


givenchy jewelry


christian louboutin


cheap jordan shoes


fitflops sandals


michael kors outlet online


air jordan shoes


michael kors outlet clearance


coach factory outlet


kate spade handbags


swarovski crystal


nike shoes on sale


nike nba jerseys


coach factory outlet


ralph lauren


bally shoes


jordan shoes


air jordan shoes


lacoste outlet


cheap michael kors handbags


supreme shirts


pandora charms


christian louboutin sale


polo ralph lauren outlet


mac outlet


pandora charms sale clearance


huf clothing


canada goose outlet store


ray ban sunglasses outlet


fingerlings monkey


kate spade purses


true religion jeans


jimmy choo sunglasses


oakley sunglasses sale


tory burch outlet store


ray ban sunglasses outlet


kate spade sale


longchamp pas cher


oakley sunglasses wholesale


coach outlet online


moncler coats


michael kors outlet clearance


basketball sneakers


michael kors


kate spade handbags


canada goose outlet online


polo ralph lauren


canada goose


jordan shoes


coach outlet online coach factory outlet


tory burch outlet online


audemars piguet watches


michael kors outlet clearance


gucci outlet online


air jordan shoes


uggs canada


cheap oakley sunglasses


nike revolution


armani exchange


gentle monster sunglasses


air jordan shoes


givenchy handbags


michael kors outlet


uggs outlet


christian louboutin


ugg boots


canada goose jackets


kate spade handbags


jordan shoes


49ers jerseys


christian louboutin sale


pandora jewelry


coach outlet store online


canada goose outlet store

yaoxuemei yaoxuemei
4 months ago

adidas yeezy shoes


coach outlet


nike kyrie 3


pandora outlet


ray ban sunglasses


coach outlet canada


cheap nfl jerseys


michael kors outlet


polo outlet


michael kors outlet


oakley sunglasses wholesale


pandora jewelry


ralph lauren outlet


cheap mlb jerseys


christian louboutin outlet


ralph lauren outlet


coach outlet


michael kors outlet


ralph lauren outlet


polo ralph lauren


gucci outlet


oakley sunglasses wholesale


fitflops sale clearance


canada goose outlet


ray ban sunglasse


fred perry polo shirts


christian louboutin shoes


coach outlet


canada goose outlet


polo outlet


polo outlet


tory burch outlet


fred perry outlet


fitflops sale clearance


coach outlet


coach outlet


michael kors outlet


off white clothing


michael kors outlet


oakley sunglasses wholesale


cheap ray bans sunglasses


canada goose outlet


polo outlet


canada goose jackets


nike air max


ralph lauren polo shirts


mcm handbags


giuseppe zanotti outlet


michael kors outlet


cheap jordans


michael kors outlet


michael kors outlet


canada goose outlet


adidas yeezy


true religion jeans


fred perry polo


coach outlet


coach outlet


pandora jewelry


pandora jewelry


uggs outlet


canada goose outlet


louboutin shoes


birkenstock shoes


canada goose outlet


oakley sunglasses


true religion jeans


ugg outlet


adidas outlet


coach outlet


kate spade handbags


mlb jerseys wholesale


christian louboutin outlet


coach outlet online


cheap nfl jerseys


michael kors outlet


ugg outlet


moncler outlet


michael kors outlet clearance


true religion jeans


christian louboutin outlet


coach outlet


true religion outlet


christian louboutin outlet


nike outlet


michael kors uk


adidas nmd


kate spade outlet


coach outlet


kate spade outlet


pandora jewelry


tory burch outlet


adidas yeezy


uggs outlet


ugg outlet


nhl jerseys


supreme uk


mont blanc pens


pandora outlet


nike shoes


coach outlet


rolex watches


michael kors handbags


nfl jerseys


kate spade outlet


superdry clothing


ray ban outlet


coach factory outlet


mbt shoes


coach outlet online


pandora outlet


nfl jerseys wholesale


coach outlet online


polo ralph lauren


adidas superstar shoes


coach outlet


uggs outlet


coach outlet


polo shirts


kate spade outlet


oakley sunglasses wholesale


ferragamo outlet


clarks shoes


michael kors outlet


coach outlet


christian louboutin outlet


coach factory outlet


pandora outlet


nike outlet


coach outlet


nfl jerseys wholesale


polo outlet


cheap nfl jerseys


cheap jordans


tory burch outlet


nba jersey


kate spade outlet


ray ban sunglasses


christian louboutin outlet


coach outlet store online


yaoxuemei20180709

yaoxuemei yaoxuemei
11 days ago

20181013 junda

canada goose parka


jordan shoes


michael kors outlet


ugg outlet


mbt shoes


coach outlet


canada goose jackets


suicoke sandals


moncler outlet


adidas nmd


swarovski outlet


pandora


nba jerseys


jordan shoes


oakley sunglasses


kings jerseys


jimmy choo shoes


kate spade outlet


michael kors outlet


bulls jerseys


christian louboutin shoes


mont blanc pens


swarovski outlet


bucks jerseys


pandora charms


soccer jerseys


michael kors handbags


oakley sunglasses


ferragamo outlet


michael kors outlet


pandora charms sale clearance


kate spade outlet


uggs outlet


ugg outlet


canada goose outlet


montblanc pens


coach outlet


coach outlet


birkenstock outlet


merrell shoes


under armour outlet


mizuno running shoes


coach outlet


fitflops


cazal sunglasses


chicago blackhawks jerseys


ugg boots


hermes outlet


fitflops outlet


hermes outlet


lebron james shoes


polo ralph lauren


harden shoes


michael kors outlet


kappa clothing


cheap jerseys


coach outlet


golden goose sneakers


nike free


real madrid jersey


air huarache


clarks shoes


canada goose outlet


michael kors outlet


magic jerseys


undefeated clothing


jordan shoes


michael kors outlet clearance


pelicans jerseys


michael kors outlet


adidas crazy


raptors jerseys


chelsea jersey


keen shoes


kate spade outlet


herve leger outlet


cheap jordans


hornets jerseys


polo ralph lauren


light up shoes


michael kors


canada goose outlet


air max


michael kors outlet


nets jerseys


mulberry handbags


michael kors outlet


polo outlet


asics shoes


christian louboutin outlet


pandora charms


ugg boots clearance


y3 shoes


uggs outlet


ugg outlet


michael kors outlet online


ugg outlet


polo shirts


san francisco 49ers jerseys


nike presto


michael kors outlet


michael kors outlet


mulberry handbags


oakley sunglasses


golden state warriors jerseys


nuggets jerseys


ugg boots clearance


nike outlet


supreme clothing


ralph lauren polo


ugg boots


true religion outlet


hawks jerseys


toms shoes


ray ban sunglasses


mulberry outlet


coach outlet


prada sunglasses


ugg outlet


coach outlet


soccer shoes


ugg boots clearance


ugg outlet


pandora jewelry


swarovski crystal


gucci outlet


nhl jerseys


football shirts


yeezy boost


ray ban sunglasses


spurs jerseys


coach factory outlet


polo ralph lauren


cheap jordans


ugg outlet


canada goose coats


red bottom shoes


trailblazers jerseys


michael kors outlet


cheap nfl jerseys


christian louboutin outlet


michael kors handbags


michael kors outlet


ugg outlet


nike factory outlet


mulberry sale


warriors jerseys


ugg outlet


kate spade outlet


ray ban sunglasses


fitflops sale clearance


canada goose outlet


louboutin pas cher


coach outlet


yeezy boost 350


moncler outlet


ugg outlet


futbol baratas


moncler outlet


fitflops sale


michael kors handbags


canada goose jackets


kate spade outlet


canada goose outlet


ugg boots


denver broncos jerseys


nike outlet


cheap jordans


mcm outlet


coach outlet online


nike blazer pas cher


michael kors outlet


nike revolution


pandora charms


canada goose jackets


canada goose outlet


camel shoes


canada goose outlet


fitflops


red bottom shoes


air jordan 3


new balance shoes


ugg outlet


canada goose jackets


red bottom shoes


columbia sportswear


ugg boots


michael kors outlet


ugg outlet


le coq sportif shoes


nike foamposite


ubiq shoes


chrome hearts sunglasses


hermes birkin


pandora outlet


oakley sunglasses


ray ban sunglasses


red bottom shoes


tory burch outlet


adidas uk


air max 2017


ray ban sunglasses


oakley sunglasses


air max 1


air huarache


ugg boots


true religion jeans


pandora charms


cheap jerseys


tods outlet


coach outlet


cheap jordans


air max 97


knicks jerseys


coach outlet


kate spade outlet


uggs outlet


coach outlet online


ed hardy clothing


lakers jerseys


pandora charms


rockets jerseys


michael kors outlet


air force 1


stussy clothing


uggs outlet


michael kors outlet


fila shoes


yeezy boost


chrome hearts outlet


suns jerseys


nike shoes


michael kors outlet


true religion jeans


ugg boots


true religion jeans


nike cortez


ferragamo outlet


mulberry handbags


polo ralph lauren


ray ban outlet


canada goose


converse shoes


swarovski crystal


celtics jerseys


michael kors outlet


cavaliers jerseys


supreme clothing


kobe shoes


tory burch outlet


ugg boots


coach outlet online


canada goose outlet


coach outlet


cleveland cavaliers jersey


ugg outlet


true religion outlet


nike shoes


polo ralph lauren


coach outlet


polo ralph lauren


five fingers shoes


burberry outlet


uggs outlet


mulberry handbags


canada goose outlet


nike lunarglide


mbt shoes


kate spade outlet


cheap jerseys


miu miu handbags


red bottom shoes


longchamp outlet


balenciaga sneakers


true religion outlet


longchamp handbags


coach handbags


pandora charms


moncler outlet


uggs outlet


canada goose outlet


kobe bryant shoes


lacoste soldes


ralph lauren shirts


michael kors outlet


christian louboutin outlet


pandora jewelry


polo outlet


kate spade outlet


michael kors wallets


canada goose jackets


uggs outlet


coach outlet


bottega veneta outlet


canada goose outlet


thunder jerseys


canada goose jackets


nike air force 1


ugg outlet


adidas wings


dior outlet


christian louboutin outlet


kate spade outlet


canada goose outlet


jordan shoes


burberry outlet


coach outlet online


coach outlet online


hermes belt


off white


michael kors outlet


polo ralph lauren


canada goose jackets


tory burch outlet


kate spade outlet


nike shoes


dc shoes


christian louboutin shoes


wizards jerseys


canada goose outlet


polo ralph lauren


fred perry polo shirts


air max 90


true religion jeans


jordan shoes


jeep shoes


michael kors


canada goose outlet


jordan shoes


polo ralph lauren


giuseppe zanotti outlet


mulberry outlet


denver broncos jerseys


baltimore ravens jerseys


uggs outlet


supra shoes


north face jackets


tory burch outlet


marc jacobs outlet


coach outlet


canada goose outlet


ralph lauren pas cher


ralph lauren pas cher


ray ban sunglasses


air jordan 4


polo outlet


cheap jordans


adidas trainers


ysl outlet


christian louboutin outlet


cheap jordans


ferragamo shoes


pacers jerseys


air jordan 4


christian louboutin store


true religion jeans


gucci outlet


longchamp bags


true religion jeans


ray ban sunglasses


michael kors


oakley sunglasses


michael kors handbags


adidas outlet


ralph lauren polo shirts


salomon shoes


dansko shoes


canada goose outlet


coach outlet


longchamp pliage


michael kors outlet


coach handbags


michael kors outlet clearance


pandora outlet


christian louboutin outlet


christian louboutin outlet


pandora outlet


michael kors outlet


swarovski outlet


coach outlet


pistons jerseys


michael kors outlet


canada goose jackets


polo outlet


gucci outlet


stephen curry shoes


world cup jerseys


jordan shoes


oakley sunglasses


nike store uk


ray ban sunglasses


nobis outlet


ferragamo outlet


van cleef & arpels jewelry


76ers jerseys


coach outlet


coach factory outlet


tory burch outlet


canada goose outlet


fitflops sale clearance


air jordan release dates


jazz jerseys


burberry outlet


coach outlet


fitflops sale clearance


san antonio spurs jerseys


tory burch outlet


nba jerseys


pandora charms


christian louboutin outlet


adidas outlet


cheap nhl jerseys


nike revolution


ugg outlet


dolce and gabbana


air max 90


air jordan retro


mishka clothing


chrome hearts outlet


ugg outlet


longchamp solde


ugg boots


mont blanc pens


pandora charms


coach outlet online


nike air max 2015


ferragamo shoes


coach outlet


coach factory outlet


longchamp handbags


polo ralph lauren


uggs outlet


coach outlet


coach outlet online


pandora charms


north face jackets


palladium boots


oakley sunglasses


gucci outlet


canada goose coats


basketball shoes


polo ralph lauren


prada shoes


barbour jackets


christian louboutin outlet


ugg outlets


canada goose outlet


birkenstock sandals


jordan shoes


moncler outlet


reebok trainers


coach outlet


heat jerseys


nfl jersey wholesale


ugg outlet


nhl jerseys


true religion jeans


coach outlet online


off-white


michael kors outlet


mulberry bags


michael kors outlet


tory burch handbags


ray ban sunglasses


nike shoes


cheap mlb jerseys


ferragamo outlet


simulation shoes


canada goose outlet


cartier jewelry


balenciaga sneakers


canada goose jackets


coach outlet


uggs


canada goose jackets


christian louboutin outlet


michael kors outlet online


clippers jerseys


canada goose jackets


michael kors outlet


canada goose coats


cheap jordans


gucci handbags


true religion uk


coach outlet


mbt shoes


christian louboutin outlet


christian louboutin outlet


supreme clothing


canada goose outlet


nike tennis


timberwolves jerseys


scarpe mbt


longchamp solde


ray ban sunglasses


canada goose outlet


michael kors


mavericks jerseys


canada goose outlet


true religion jeans


coach outlet


cheap jordans


adidas nmd


yeezy boost


pandora charms


lakers jerseys


coach outlet online


valentino outlet


manchester united jersey


air jordan shoes


toms shoes


cheap jordans


coach outlet


ralph lauren polo shirts


minnetonka outlet


reebok outlet store


longchamp outlet


canada goose jackets


new york knicks jersey


canada goose coats


pandora charms


celine outlet


cheap mlb jerseys


true religion jeans


ugg boots


canada goose outlet


cheap jordans


ugg outlet


uggs outlet


jack wolfskin


christian louboutin outlet


christian louboutin boutique


michael kors handbags


ugg boots


uggs outlet


mulberry bags


adidas ultra boost


vans shoes


fitflops shoes


ugg outlet


soccer jerseys


michael kors outlet


polo ralph lauren


bvlgari jewelry


pandora jewelry


ugg outlet


tory burch outlet


oakley sunglasses


canada goose outlet


longchamp handbags


moncler jackets


coach canada


cheap snapbacks


ferragamo outlet


coach outlet


tory burch outlet


visvim shoes


michael kors outlet


moncler jackets


kobe 9


lacoste polo shirts


nike roshe one


coach outlet


nfl jerseys wholesale


ugg boots


michael kors outlet


north face jackets


ugg outlet


tory burch outlet


manolo blahnik outlet


air jordan shoes


cheap football shirts


ray ban sunglasses


true religion jeans


ralph lauren polo shirts


christian louboutin outlet


fitflops sale


toms shoes


nike dunks


cheap jordans


uggs outlet


moncler outlet


supreme clothing


moncler outlet


louboutin shoes


coach outlet online


michael kors outlet


saucony shoes


swarovski outlet


polo ralph lauren


houston texans jerseys


true religion jeans


canada goose outlet


longchamp outlet


barcelona jersey


polo outlet


puma outlet


ralph lauren outlet


indianapolis colts jerseys


nba jerseys


wellensteyn jackets


air max 90


coach outlet


coach outlet store online


michael kors outlet


ralph lauren polo


jordan shoes


grizzlies jerseys


michael kors outlet


ugg boots


malone souliers mules


kate spade outlet


cheap nfl jerseys


burberry outlet


canada goose outlet


true religion outlet


coach outlet


kate spade outlet


canada goose jackets


nike shoes


givenchy handbags


kevin durant shoes


air jordan 12


prada outlet


canada goose jackets


cheap jordans


links of london


ecco shoes


cheap jordans


moncler outlet

Image1517971597?1517971597
Round: Peer Prize Round
Category: Default

Votes

Ranking

Researcher

62

1

Recent Voters